Regulatory

Part:BBa_K3675001:Design

Designed by: Tiago Lopes   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 84
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 607
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 314


Design Notes

The sequence of this promoter was obtained by the annalysis of the region 2000bp upstream of the coding sequence of the gene enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase by the use of the online tool BPROM from softberry (http://www.softberry.com/berry.phtml). Additionally to this sequence was included the Shine-Delgarno sequence. In order to make this part compatible with RCF[10] we plan to make a modification near the position 606 to remove the restriction site incompatible with the assembly standard.


Source

The sequence was obtained using the tool softberry (http://www.softberry.com/berry.phtml).The original sequence used for this analysis was retrieved from GenBank.

References